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Image Search Results
Journal: STAR Protocols
Article Title: Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae
doi: 10.1016/j.xpro.2022.101900
Figure Lengend Snippet: MatlabTrack GUI for single-molecule tracking The various modules are bordered in red and numbered according to their sequence. 1. Press this button for loading the .tiff file (a time-lapse single-molecule movie), 2. Filter the images for denoising using Bandpass filter, 3. Create ROIs against your area of interest, where you want to track the single molecules (such as nucleus, spindle, transcription site visualized by GFP channel). A prompt will appear upon clicking “Add ROI” to load the reference image. 4. Detect particles using intensity thresholding, 5. Track the particles frame-to-frame to generate trajectories, 6. Manually check the tracks, delete, or edit, if necessary, 7. Analyze the tracks. Save the results as .mat file from the “File” menu.
Article Snippet:
Techniques: Sequencing
Journal: STAR Protocols
Article Title: Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae
doi: 10.1016/j.xpro.2022.101900
Figure Lengend Snippet: MatlabTrack GUI for manually verifying the tracks (A) Using this window, you can visually verify the automated tracking performed by the software. You can also edit or delete or merge the tracks if required. (B) Example of a track that do not need any manual editing. (C) Example of tracks that we manually add and merge to make a single long track out of four short tracks. (D) Example of tracks that we manually delete.
Article Snippet:
Techniques: Software
Journal: STAR Protocols
Article Title: Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae
doi: 10.1016/j.xpro.2022.101900
Figure Lengend Snippet: MatlabTrack GUI for analyzing survival time distribution The left curve shows the photobleaching kinetics. The right curve shows the survival time distribution. You can checkmark the “Photobleaching Correction” box to normalize the survival time distribution with the photobleaching kinetics. Based on the fitting of the survival time distribution (either single exponential fit or double exponential fit), the fraction of bound molecules has been calculated as “f1”. The inverse of the k off value represents the residence time. Click on the “Tools” menu and select “Generate Pie Chart” option to see the fraction of bound molecules with their residence time. The “total tracks” (red rectangle) should be generally >1000 for better statistical significance.
Article Snippet:
Techniques:
Journal: STAR Protocols
Article Title: Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae
doi: 10.1016/j.xpro.2022.101900
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Imaging, Diagnostic Assay, Software, Control, Microscopy